Competition between Li+ and Mg2+ for the phosphate groups in the human erythrocyte membrane and ATP: an NMR and fluorescence study.

@article{Freitas1994CompetitionBL,
  title={Competition between Li+ and Mg2+ for the phosphate groups in the human erythrocyte membrane and ATP: an NMR and fluorescence study.},
  author={Duarte E Mota de Freitas and Louis Amari and Chandra Srinivasan and Qinfen Rong and Ramaraja Ramasamy and Aron Abraha and Carlos F.G.C. Geraldes and Mary K. Boyd},
  journal={Biochemistry},
  year={1994},
  volume={33 14},
  pages={4101-10}
}
We investigated the mechanism of competition between Li+ and Mg2+ in Li(+)-loaded human red blood cells (RBCs) by making 7Li and 31P NMR and fluorescence measurements. We used 7Li NMR relaxation times to probe Li+ binding to the human RBC membrane and ATP; an increase in Mg2+ concentration caused an increase in both 7Li T1 and T2 values in packed Li(+)-loaded RBCs, in suspensions of Li(+)-loaded RBC ghosts, in suspensions of Li(+)-containing RBC membrane, and in aqueous solutions of ATP… CONTINUE READING

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