Comparisons of oocyte maturation times and of three methods of sperm preparation for their effects on the production of goat embryos in vitro.

@article{Rho2001ComparisonsOO,
  title={Comparisons of oocyte maturation times and of three methods of sperm preparation for their effects on the production of goat embryos in vitro.},
  author={Gyu Jin Rho and Ann C Hahnel and Keith J Betteridge},
  journal={Theriogenology},
  year={2001},
  volume={56 3},
  pages={503-16}
}
Various times of in vitro maturation of oocytes, and three methods of separating spermatozoa from frozen-thawed semen (Percoll density-gradient centrifugation, swim-up, and glass-wool filtration), were compared for their effects on goat embryo production in vitro. Cumulus-oocyte-complexes (COCs) from abattoir ovaries were matured in M199 supplemented with 10% fetal calf serum and hormones. In Experiment 1, COCs were fixed at 4 h intervals from 0 to 27 h of culture to assess oocyte nuclear… CONTINUE READING

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In Experiment 4 , embryos were compared for cleavage at 48 h and development into blastocysts , hatching rates and cell number at 192 h. The rates of cleavage and blastocyst formation in the Percoll - treated group were higher ( P < 0.05 ) than in the swim - up and glass - wool groups ( 62% and 18% vs. 50% and 11% , and 45% and 8% , respectively ) .
In Experiment 4 , embryos were compared for cleavage at 48 h and development into blastocysts , hatching rates and cell number at 192 h. The rates of cleavage and blastocyst formation in the Percoll - treated group were higher ( P < 0.05 ) than in the swim - up and glass - wool groups ( 62% and 18% vs. 50% and 11% , and 45% and 8% , respectively ) .
In Experiment 4 , embryos were compared for cleavage at 48 h and development into blastocysts , hatching rates and cell number at 192 h. The rates of cleavage and blastocyst formation in the Percoll - treated group were higher ( P < 0.05 ) than in the swim - up and glass - wool groups ( 62% and 18% vs. 50% and 11% , and 45% and 8% , respectively ) .
In Experiment 4 , embryos were compared for cleavage at 48 h and development into blastocysts , hatching rates and cell number at 192 h. The rates of cleavage and blastocyst formation in the Percoll - treated group were higher ( P < 0.05 ) than in the swim - up and glass - wool groups ( 62% and 18% vs. 50% and 11% , and 45% and 8% , respectively ) .
In Experiment 4 , embryos were compared for cleavage at 48 h and development into blastocysts , hatching rates and cell number at 192 h. The rates of cleavage and blastocyst formation in the Percoll - treated group were higher ( P < 0.05 ) than in the swim - up and glass - wool groups ( 62% and 18% vs. 50% and 11% , and 45% and 8% , respectively ) .
In Experiment 4 , embryos were compared for cleavage at 48 h and development into blastocysts , hatching rates and cell number at 192 h. The rates of cleavage and blastocyst formation in the Percoll - treated group were higher ( P < 0.05 ) than in the swim - up and glass - wool groups ( 62% and 18% vs. 50% and 11% , and 45% and 8% , respectively ) .
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