Comparison of yoghurt, heat treated yoghurt, milk and lactose effects on plasmid dissemination in gnotobiotic mice

  title={Comparison of yoghurt, heat treated yoghurt, milk and lactose effects on plasmid dissemination in gnotobiotic mice},
  author={Sylvie Maisonneuve and M F Ouriet and Yvonne Duval-Iflah},
  journal={Antonie van Leeuwenhoek},
The effect of yoghurt, heat-treated fermented milk, milk and lactose solution intake on plasmid transfer and establishment of the resulting transconjugants in the digestive tract of mice colonised with human faecal flora were examined. Yoghurt lowered the population level of transconjugants more efficiently than heat-treated fermented milk (−2 log and −1 log respectively) and indicated a beneficial effect of viable bacteria. On the other hand consumption of milk drastically inhibited the… 
Nutritional value of yoghurt
The chemical composition of a foodstuff provides a useful indication of its potential nutritional value. This chapter illustrates the main components of some typical natural and fruit yoghurts. It is
Carica papaya seed macerate as inhibitor of conjugative R plasmid transfer from Salmonella typhimurium to Escherichia coli in vitro and in the digestive tract of gnotobiotic mice.
The data obtained show a clinical potential use of papaya seed macerate on conjugal R plasmid transfer from Salmonella typhimurium to Escherichia coli, and strategies to reduce or minimize the dissemination of these genes could be relevant.
Interference of Lactobacillus plantarum Strains in the In Vitro Conjugative Transfer of R-Plasmids
Investigation of the in vitro capability of two Lactobacillus plantarum strains to interfere with the conjugation processes demonstrated that both lactobacilli strains were able to decrease mating frequency.
Nutrition Related Stress Factors Reduce the Transfer of Extended-Spectrum Beta-Lactamase Resistance Genes between an Escherichia coli Donor and a Salmonella Typhimurium Recipient In Vitro
It was showed, for the model mating pair, that conjugation frequencies decreased under different physiological stress conditions, and the hypothesis that stress factors may enhance conjugations should be viewed with caution.
Usefulness of an experimental model of the infant gut.
  • M. Butel
  • Biology, Medicine
    Journal of pediatric gastroenterology and nutrition
  • 2003
The autochthonous intestinal microflora is believed to play a major role in colonization resistance by preventing overgrowth of potential pathogens and beneficial effects on the mucosal immune system were shown through immunomodulation by bifidobacteria in vitro and in experimental models.
The relevance of gene transfer to the safety of food and feed derived from genetically modified ( GM ) plants
HGT is reviewed in relation to the potential risks of consuming food or feed derived from transgenic crops and the results of the deliberations are laid down in this review paper.
The relevance of gene transfer to the safety of food and feed derived from genetically modified (GM) plants.
  • G. van den Eede, H. Aarts, A. Wilcks
  • Biology
    Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
  • 2004
Horizontal gene transfer amongst probiotic lactic acid bacteria and other intestinal microbiota: what are the possibilities? A review
In this review, the possible factors that may be exchanged and the methods of exchange are discussed.
Lactobacillus GG prevents respiratory infections in children : a convincing study
It is usual and surprising to record that persons who consume again milk products do not suffer from discomfort despite the lack of β-galactosidase activity, which is confirmed by intestinal biopsy.
Dietary Factors that Affect Gut Health and Pathogen Colonization
A ventilated welding mask is disclosed having a three chamber housing mounted thereon, a blower fan is positioned within a second medial chamber and an air flow manifold positioned with the welding mask.


Effects of yoghurt intake on plasmid transfer and colonisation with transconjugants in the digestive tract of mice associated with human faecal flora.
It was shown that the self-transmissible plasmid R388 was efficiently transferred to recipient strain PG1 in mice associated with human faecal flora (HFF-PG1) and that the resulting transconjugants (PG1-R388) became established at a high and maximal population level without any selective pressure.
Effect of fermented milk intake on plasmid transfer and on the persistence of transconjugants in the digestive tract of gnotobiotic mice
The results indicate for the first time that probiotics have various effects on the formation and/or establishment of transconjugants in the gut of axenic mice, indicating that bacterial metabolites and viable bacteria can be involved.
Effect of yogurt on symptoms and kinetics of hydrogen production in lactose-malabsorbing children.
In children, mechanisms other than enhanced lactose absorption from yogurt may lead to changes in the kinetics of hydrogen production, which in turn are associated with improved tolerance, and this effect and its mechanisms in children are compared.
Effect of the microbial lactase (EC activity in yoghurt on the intestinal absorption of lactose: An in vivo study in lactase-deficient humans
Findings indicate that more than 90% of the lactose in yoghurt is digested in the small intestine of lactase-deficient subjects and suggest that both the lactase activity contained in the viable starter culture and a slow oro-caecal transit time are responsible for this excellent absorption.
Quantitative Changes in Sugars, Especially Oligosaccharides, During Fermentation and Storage of Yogurt
Abstract Yogurt was prepared by fermentation with Lactobacillus bulgaricus and Streptococcus thermophilus . Lactose content (6.53%) of yogurt mix progressively decreased to 4.22% during fermentation.
Effect of Feeding Yogurt Versus Milk in Children with Persistent Diarrhea
Preliminary results strongly suggest a clinical advantage of feeding yogurt rather than milk in children with persistent diarrhea.
Clinical uses of probiotics for stabilizing the gut mucosal barrier: successful strains and future challenges
Probiotic bacteria appear to be promising candidates for the treatment of clinical conditions with abnormal gut microflora and altered gut mucosal barrier functions and promising ingredients to future functional foods and clinical foods for specific disease states provided that basic requirements for strains and clinical studies are carefully followed.
Transfer of the shuttle vector pRRI207 between Escherichia coli and Bacteroides spp. in vitro and in vivo in the digestive tract of axenic mice and in gnotoxenic mice inoculated with a human microflora
Transfer of the shuttle vector pRRI207 mediated by the helper plasmid pRK2013 from Escherichia coli to Bacteroides spp. was possible in vitro and in vivo in the digestive tract of axenic mice
Recombinant DNA transfer to Escherichia coli of human faecal origin in vitro and in digestive tract of gnotobiotic mice
This work indicates that ≥ 50% of wild-type E. coli strains were able to promote transfer of pBR oriT− plasmids in vitro, and that transconjugants ofWild-type strains obtained in in vitro mating experiments and inoculated into gnotobiotic HFF mice were eliminated as rapidly as the recombinant K12 strains.
Tetracycline-dependent appearance of plasmidlike forms in Bacteroides uniformis 0061 mediated by conjugal Bacteroides tetracycline resistance elements
Neither NBU1 or NBU2 could be detected as a plasmidlike form in cells exposed to UV light, thymidine starvation, mitomycin C, or autoclaved chlortetracycline (50 micrograms/ml).