Comparison of yoghurt, heat treated yoghurt, milk and lactose effects on plasmid dissemination in gnotobiotic mice

  title={Comparison of yoghurt, heat treated yoghurt, milk and lactose effects on plasmid dissemination in gnotobiotic mice},
  author={Sylvie Maisonneuve and M F Ouriet and Yvonne Duval-Iflah},
  journal={Antonie van Leeuwenhoek},
The effect of yoghurt, heat-treated fermented milk, milk and lactose solution intake on plasmid transfer and establishment of the resulting transconjugants in the digestive tract of mice colonised with human faecal flora were examined. Yoghurt lowered the population level of transconjugants more efficiently than heat-treated fermented milk (−2 log and −1 log respectively) and indicated a beneficial effect of viable bacteria. On the other hand consumption of milk drastically inhibited the… 

Interrelationships between dairy product intake, microflora metabolism, faecal properties and plasmid dissemination in gnotobiotic mice

The main modifications were the reduction of population levels of transconjugants in mice receiving yoghurt, HTY and milk, and concomitantly an increase ofβ-galactosidase and a decrease of β-glucosid enzyme activities, compared with control mice fed a standard diet.

Nutritional value of yoghurt

The chemical composition of a foodstuff provides a useful indication of its potential nutritional value. This chapter illustrates the main components of some typical natural and fruit yoghurts. It is

Interference of Lactobacillus plantarum Strains in the In Vitro Conjugative Transfer of R-Plasmids

Investigation of the in vitro capability of two Lactobacillus plantarum strains to interfere with the conjugation processes demonstrated that both lactobacilli strains were able to decrease mating frequency.

Inhibitory Impact of Bifidobacteria on the Transfer of β-Lactam Resistance among Enterobacteriaceae in the Gnotobiotic Mouse Digestive Tract

Bifidobacteria appear to have an inhibitory impact on the transfer of antibiotic resistance genes and the inhibitory effect is associated to specific b ifidobacterial strains and may be related to the production of thermostable metabolites by these strains.

Nutrition Related Stress Factors Reduce the Transfer of Extended-Spectrum Beta-Lactamase Resistance Genes between an Escherichia coli Donor and a Salmonella Typhimurium Recipient In Vitro

It was showed, for the model mating pair, that conjugation frequencies decreased under different physiological stress conditions, and the hypothesis that stress factors may enhance conjugations should be viewed with caution.

Usefulness of an experimental model of the infant gut.

  • M. Butel
  • Biology, Medicine
    Journal of pediatric gastroenterology and nutrition
  • 2003
The autochthonous intestinal microflora is believed to play a major role in colonization resistance by preventing overgrowth of potential pathogens and beneficial effects on the mucosal immune system were shown through immunomodulation by bifidobacteria in vitro and in experimental models.

Controlling Gut Health without the Use of Antibiotics

During the past 50 years, the livestock and poultry industries have developed in several areas including nutrition, genetics, engineering, management, and communications to maximizing the efficiency

The relevance of gene transfer to the safety of food and feed derived from genetically modified ( GM ) plants

HGT is reviewed in relation to the potential risks of consuming food or feed derived from transgenic crops and the results of the deliberations are laid down in this review paper.

The relevance of gene transfer to the safety of food and feed derived from genetically modified (GM) plants.

  • G. van den EedeH. Aarts A. Wilcks
  • Biology
    Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
  • 2004

Horizontal gene transfer amongst probiotic lactic acid bacteria and other intestinal microbiota: what are the possibilities? A review

In this review, the possible factors that may be exchanged and the methods of exchange are discussed.



Effects of yoghurt intake on plasmid transfer and colonisation with transconjugants in the digestive tract of mice associated with human faecal flora.

It was shown that the self-transmissible plasmid R388 was efficiently transferred to recipient strain PG1 in mice associated with human faecal flora (HFF-PG1) and that the resulting transconjugants (PG1-R388) became established at a high and maximal population level without any selective pressure.

Effect of fermented milk intake on plasmid transfer and on the persistence of transconjugants in the digestive tract of gnotobiotic mice

The results indicate for the first time that probiotics have various effects on the formation and/or establishment of transconjugants in the gut of axenic mice, indicating that bacterial metabolites and viable bacteria can be involved.

Effect of yogurt on symptoms and kinetics of hydrogen production in lactose-malabsorbing children.

In children, mechanisms other than enhanced lactose absorption from yogurt may lead to changes in the kinetics of hydrogen production, which in turn are associated with improved tolerance, and this effect and its mechanisms in children are compared.

Viable starter culture, beta-galactosidase activity, and lactose in duodenum after yogurt ingestion in lactase-deficient humans.

Data clearly show that after fresh yogurt ingestion, viable starter culture reaches the duodenum and contains beta-galactosidase activity, however, the buffering capacity of the yogurt that protects bacteria from acidic gastric secretion also prevents microbial beta-GalactOSidase from hydrolyzing lactose in theduodenum.

Effect of the microbial lactase (EC activity in yoghurt on the intestinal absorption of lactose: An in vivo study in lactase-deficient humans

Findings indicate that more than 90% of the lactose in yoghurt is digested in the small intestine of lactase-deficient subjects and suggest that both the lactase activity contained in the viable starter culture and a slow oro-caecal transit time are responsible for this excellent absorption.

Splenic and intestinal lymphocyte proliferation response in mice fed milk or yogurt and challenged with Salmonella typhimurium.

An improvement in local gastrointestinal as well as systemic immunity on a yogurt diet as compared to a milk diet is indicated, and Serum IgA levels in Salmonella-challenged mice were significantly higher 8 days after the challenge in the yogurt- fed group than in the milk-fed group.

Quantitative Changes in Sugars, Especially Oligosaccharides, During Fermentation and Storage of Yogurt

Abstract Yogurt was prepared by fermentation with Lactobacillus bulgaricus and Streptococcus thermophilus . Lactose content (6.53%) of yogurt mix progressively decreased to 4.22% during fermentation.

Antagonisms among isogenic strains of Escherichia coli in the digestive tracts of gnotobiotic mice

Maintaining the bacterial strains in monoassociation with gnotobiotic mice resulted in the "adaptation" of the bacteria to their host, and in all cases, "adapted" strains became the dominant population in the feces of mice, regardless of whether the adapted strains was introduced into mice before or after its isogenic partner which had been cultured in vitro.

Effect of Feeding Yogurt Versus Milk in Children with Persistent Diarrhea

Preliminary results strongly suggest a clinical advantage of feeding yogurt rather than milk in children with persistent diarrhea.

Probiotics in man and animals.

  • R. Fuller
  • Biology, Medicine
    The Journal of applied bacteriology
  • 1989
These are all areas where the gut flora can be altered for the worse and where, by the administration of probiotics, the natural balance of the gut microflora can be restored and the animal returned to its normal nutrition, growth and health status.