Comparison of weak affinity chromatography and surface plasmon resonance in determining affinity of small molecules.

Abstract

In this study, we compared affinity data from surface plasmon resonance (SPR) and weak affinity chromatography (WAC), two established techniques for determination of weak affinity (mM-μM) small molecule-protein interactions. In the current comparison, thrombin was used as target protein. In WAC the affinity constant (KD) was determined from retention times… (More)
DOI: 10.1016/j.ab.2014.05.023

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Cite this paper

@article{DuongThi2014ComparisonOW, title={Comparison of weak affinity chromatography and surface plasmon resonance in determining affinity of small molecules.}, author={Minh-Dao Duong-Thi and Gunnar Bergstr{\"o}m and Carl-Fredrik Mandenius and M Andresen Bergstr{\"o}m and Tomas Fex and Sten Ohlson}, journal={Analytical biochemistry}, year={2014}, volume={461}, pages={57-9} }