Comparison of typing results by serology and polymerase chain reaction with sequence-specific primers for HLA-Cw in 650 individuals.

@article{Mytilineos1997ComparisonOT,
  title={Comparison of typing results by serology and polymerase chain reaction with sequence-specific primers for HLA-Cw in 650 individuals.},
  author={Joannis Mytilineos and U. Christ and Małgorzata Lempert and Gerhard Opelz},
  journal={Tissue antigens},
  year={1997},
  volume={50 4},
  pages={395-400}
}
HLA-Cw typing by standard serological techniques is associated with a high frequency of blanks, and reliable typing reagents for several of the Cw specificities are scarce. We evaluated the PCR-SSP technique for Cw typing in 370 kidney transplant patients and 280 healthy blood donors. Serological typing of all individuals was performed in our laboratory from 1995 to 1997 using commercially available tissue-typing trays. Comparison between serological and PCR-SSP typing revealed a discrepancy… CONTINUE READING
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