Comparison of two methods for obtaining spermatozoa from the cauda epididymis of Iberian red deer.

@article{MartnezPastor2006ComparisonOT,
  title={Comparison of two methods for obtaining spermatozoa from the cauda epididymis of Iberian red deer.},
  author={F Mart{\'i}nez-Pastor and Vanesa Garc{\'i}a-Mac{\'i}as and Mercedes Prudencio {\'A}lvarez and Cesar A Chamorro and Paz Herr{\'a}ez and P L P{\'e}rez de Paz and Luis Anel},
  journal={Theriogenology},
  year={2006},
  volume={65 3},
  pages={471-85}
}
We have compared two methods for salvaging epididymal sperm from post-mortem samples from Iberian red deer. Of each pair of testicles (29 samples), one cauda epididymis was processed by means of cuts (sperm was immediately diluted with extender) and the other was detached from the corpus and flushed from the vas deferens with 1 mL of extender. Sperm was processed for cryopreservation, and analyzed just after recovery, pre-freezing and post-thawing. Total spermatozoa recovered, contamination… CONTINUE READING

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