Comparison of two enzyme-linked immunosorbent assays for antigen quantitation: direct competition and antibody inhibition.

Abstract

Two enzyme-linked immunosorbent assays for antigen quantitation, direct competition and antibody inhibition, were used to measure rabbit immunoglobulin G in polystyrene microtiter plates and were compared for sensitivity and reproducibility. Both procedures repetitively detected this antigen in the 1- to 100-ng/ml range. Both procedures were predictably… (More)

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