Comparison of two bacterial azoreductases acquired during adaptation to growth on azo dyes

@article{Zimmermann1984ComparisonOT,
  title={Comparison of two bacterial azoreductases acquired during adaptation to growth on azo dyes},
  author={Thomas von Zimmermann and Franz Gasser and Hans G. Kulla and Thomas Leisinger},
  journal={Archives of Microbiology},
  year={1984},
  volume={138},
  pages={37-43}
}
Selection for utilization of carboxy-Orange I [1-(4′-carboxyphenylazo)-4-naphthol] in the chemostat yielded Pseudomonas strain K24 which was unable to grow on carboxy-Orange II [1-(4′-carboxyphenylazo)-2naphthol] while selection for growth on carboxy-Orange II had previously led to strain KF 46 which did not utilize carboxy-Orange I. Orange I azoreductase of strain K24, the key enzyme of dye degradation, was purified 80-fold with 17% yield to electrophoretic homogeneity and compared to the… CONTINUE READING
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