Comparison of toxicogenomics and traditional approaches to inform mode of action and points of departure in human health risk assessment of benzo[a]pyrene in drinking water.

Abstract

Toxicogenomics is proposed to be a useful tool in human health risk assessment. However, a systematic comparison of traditional risk assessment approaches with those applying toxicogenomics has never been done. We conducted a case study to evaluate the utility of toxicogenomics in the risk assessment of benzo[a]pyrene (BaP), a well-studied carcinogen, for drinking water exposures. Our study was intended to compare methodologies, not to evaluate drinking water safety. We compared traditional (RA1), genomics-informed (RA2) and genomics-only (RA3) approaches. RA2 and RA3 applied toxicogenomics data from human cell cultures and mice exposed to BaP to determine if these data could provide insight into BaP's mode of action (MOA) and derive tissue-specific points of departure (POD). Our global gene expression analysis supported that BaP is genotoxic in mice and allowed the development of a detailed MOA. Toxicogenomics analysis in human lymphoblastoid TK6 cells demonstrated a high degree of consistency in perturbed pathways with animal tissues. Quantitatively, the PODs for traditional and transcriptional approaches were similar (liver 1.2 vs. 1.0 mg/kg-bw/day; lungs 0.8 vs. 3.7 mg/kg-bw/day; forestomach 0.5 vs. 7.4 mg/kg-bw/day). RA3, which applied toxicogenomics in the absence of apical toxicology data, demonstrates that this approach provides useful information in data-poor situations. Overall, our study supports the use of toxicogenomics as a relatively fast and cost-effective tool for hazard identification, preliminary evaluation of potential carcinogens, and carcinogenic potency, in addition to identifying current limitations and practical questions for future work.

DOI: 10.3109/10408444.2014.973934
0200400600201520162017
Citations per Year

585 Citations

Semantic Scholar estimates that this publication has 585 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Moffat2015ComparisonOT, title={Comparison of toxicogenomics and traditional approaches to inform mode of action and points of departure in human health risk assessment of benzo[a]pyrene in drinking water.}, author={Ivy D. Moffat and Nikolai L. Chepelev and Sarah Labib and Julie A Bourdon-Lacombe and Byron Kuo and Julie K. Buick and France Lemieux and Andrew Williams and Sabina Halappanavar and Amal Idris Malik and Mirjam Luijten and Jiri Aubrecht and Daniel R. Hyduke and Albert J. Fornace and Carol D. Swartz and Leslie Recio and Carole Yauk}, journal={Critical reviews in toxicology}, year={2015}, volume={45 1}, pages={1-43} }