Comparison of three nonradioisotopic polymerase chain reaction-based methods for detection of human immunodeficiency virus type 1.

@article{Whetsell1992ComparisonOT,
  title={Comparison of three nonradioisotopic polymerase chain reaction-based methods for detection of human immunodeficiency virus type 1.},
  author={Amy J Whetsell and Jana Brittain Drew and G E Milman and Rodney Hoff and Elizabeth Dragon and Kristin Adler and Jennie Hui and Peter Otto and Phalguni Gupta and Homayoon Farzadegan},
  journal={Journal of clinical microbiology},
  year={1992},
  volume={30 4},
  pages={845-53}
}
Three nonradioisotopic polymerase chain reaction (PCR)-based detection techniques were evaluated for sensitivity and specificity in detecting human immunodeficiency virus type 1 (HIV-1) proviral DNA in peripheral blood mononuclear cells. The Roche prototype HIV-1 PCR assay, the Du Pont enzyme-linked oligonucleotide sandwich assay (ELOSA), and the Gen-Probe hybridization protection assay (HPA) were compared with a standard radioisotopic oligonucleotide solution hybridization (OSH) technique. A… CONTINUE READING

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