Comparison of three methods for cryopreservation of human embryonic stem cells.

@article{Li2010ComparisonOT,
  title={Comparison of three methods for cryopreservation of human embryonic stem cells.},
  author={Yang Li and Ji-chun Tan and Ling-song Li},
  journal={Fertility and sterility},
  year={2010},
  volume={93 3},
  pages={999-1005}
}
OBJECTIVE To establish reliable methods for cryopreservation of human embryonic stem cells (hESCs). DESIGN Prospective experimental study. SETTING University laboratory. PATIENT(S) One hESC line. INTERVENTION(S) The attachment rates and recovery rates of cryopreserved hESCs using three different cryopreservation methods were compared. MAIN OUTCOME MEASURE(S) The hESCs were frozen and thawed by conventional cryopreservation, programmable cryopreservation, and vitrification method. The… CONTINUE READING