Comparison of the toxins of the blue-green alga Aphanizomenon flos-aquae with the Gonyaulax toxins.

@article{Ikawa1982ComparisonOT,
  title={Comparison of the toxins of the blue-green alga Aphanizomenon flos-aquae with the Gonyaulax toxins.},
  author={Miyoshi Ikawa and Konrad Wegener and Thomas L. Foxall and John J. Sasner},
  journal={Toxicon : official journal of the International Society on Toxinology},
  year={1982},
  volume={20 4},
  pages={747-52}
}
A toxic strain of Aphanizomenon flos-aquae (NH-1), isolated from a toxic bloom in a pond in Durham, New Hampshire, has been mass cultured in the laboratory. The toxin was extracted by ultrasonic disruption of the cells and purified by; (a) filtration through a 10 kilodalton filter, and (b) chromatography on a strong cation exchange resin column using 0.01 M, then 0.1 M, pH 5, sodium acetate buffer followed by 0.75 M hydrochloric acid. Mouse assays and fluorescence generated by hydrogen peroxide… CONTINUE READING