Comparison of the sensitivity of culture, PCR and quantitative real-time PCR for the detection of Pseudomonas aeruginosa in sputum of cystic fibrosis patients

@inproceedings{Deschaght2009ComparisonOT,
  title={Comparison of the sensitivity of culture, PCR and quantitative real-time PCR for the detection of Pseudomonas aeruginosa in sputum of cystic fibrosis patients},
  author={Pieter Deschaght and Thierry de Baere and Leen Van Simaey and Sabine Van daele and Frans De Baets and Daniel De Vos and Jean-Paul Pirnay and Mario Vaneechoutte},
  booktitle={BMC Microbiology},
  year={2009}
}
Pseudomonas aeruginosa is the major pathogen involved in the decline of lung function in cystic fibrosis (CF) patients. Early aggressive antibiotic therapy has been shown to be effective in preventing chronic colonization. Therefore, early detection is important and sensitive detection methods are warranted. In this study, we used a dilution series of P. aeruginosa positive sputa, diluted in a pool of P. aeruginosa negative sputa, all from CF patients - to mimick as closely as possible the… CONTINUE READING
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References

Publications referenced by this paper.
SHOWING 1-10 OF 38 REFERENCES

Comparison of conventional and molecular methods for the detection of bacterial pathogens in sputum samples from cystic fibrosis

A Van Belkum, NHM Renders, S Smith, SE Overbeek, HA Verbrugh
  • FEMS Immunol Med Microbiol
  • 2000
VIEW 3 EXCERPTS
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