Comparison of the kinetic properties of membrane-bound and solubilized Na,K-ATPase.

@article{Boldyrev1990ComparisonOT,
  title={Comparison of the kinetic properties of membrane-bound and solubilized Na,K-ATPase.},
  author={Alexander A. Boldyrev and Natalya U. Fedosova},
  journal={Biochemistry international},
  year={1990},
  volume={22 5},
  pages={903-11}
}
Substrate-velocity curve for Na,K-ATPase under optimal conditions is described as a curve with intermediary plateau. C12E2 treatment of the enzyme changes its kinetic behaviour. The substrate-velocity curve transforms into hyperbolic one and the Km value for the solubilized enzyme approaches the Km value for the first phase of the complex curve. The experimental substrate-velocity curves obtained for Na,K-ATPase under different conditions were analyzed on the basis of the sum of Michaelis and… CONTINUE READING