• Corpus ID: 89033616

Comparison of the infectious forms of two bacterial endonucleobionts, Holospora elegans and H. obtusa, from the ciliate Paramecium caudatum

  title={Comparison of the infectious forms of two bacterial endonucleobionts, Holospora elegans and H. obtusa, from the ciliate Paramecium caudatum},
  author={Helmut J. Schmidt and Manfred Freiburg and Hans-Dieter Gortz},
Evaluation of Enrichment Protocols for Bacterial Endosymbionts of Ciliates by Real-Time PCR
This study developed a real-time PCR-based strategy and employed it for assessing two of such enrichment protocols for Holospora caryophila, hosted by the ciliate Paramecium, and concluded that the “cell fractionation” protocol was quite successful in the enrichment of the symbiont, while the ‘Percoll gradient’ protocol will need further refinements to be fully repeatable.
“Candidatus Gortzia shahrazadis”, a Novel Endosymbiont of Paramecium multimicronucleatum and a Revision of the Biogeographical Distribution of Holospora-Like Bacteria
A novel species belonging to the “Ca. Gortzia” genus, detected in P. multimicronucleatum, a ciliate for which infection by an HLB has not been reported, discovered in India is described.
18 Symbiotic Associations Between Ciliates and Prokaryotes
Genus Caedibacter, Genus Lyticum, and Genus Paraholospora are described in detail in “Generating Characters of Caesium,” a 560-year-old treatise on infectious disease and fungal infection.
Endosymbionts in paramecium.
Infection and Maintenance of Holospora Species in Paramecium caudatum
Infectious forms of Holospora species have a cytoplasmic region and a periplasmic lumen with an electron-translucent invasion tip, providing an excellent opportunity for elucidating host-symbiont interactions between eukaryotic and prokaryotic cells and establishment of new associations leading to eukARYotic cell evolution.
Endonuclear bacteria in Euglena hemichromata (Euglenophyceae): a proposed pathway to endonucleobiosis
Endonuclear bacteria were observed within Euglena hemichromata cells isolated from a bloom in a small pond in South Korea, suggesting this type of endonucleobiosis may be involved in the control of euglenoid blooms in nature.
Cell Structure of the Infectious Form of Holospora, an Endonuclear Symbiotic Bacterium of the Ciliate Paramecium
It is found that this special tip could be identified with Nomarski- and phase-contrast optics at the end of the dark region when the infectious form was embedded in 30% gelatin, and that the electrontranslucent tip should be present at the opposite end.
Cloning and Sequencing of Gene Coding for a Periplasmic 5.4 kDa Peptide of the Macronucleus-Specific Symbiont Holospom obtusa of the Ciliate Paramecium caudatum
P purified a 5.4 kDa peptide which is present in the intermediate and infectious form, but not in the reproductive form of a symbiotic bacterium Holospora obtusa of the ciliate Paramecium caudatum, and determined the transcription start point by primer extension analysis.
Interactions of Host Paramecia With Infectious Holospora Endocytobionts
Digoxigenin-labeled bacterial surface proteins were found to bind to blotted nuclear envelope proteins, indicating a possible interaction in the invasion process of Holospora obtusa.