Comparison of the human and bovine milk N-glycome via high-performance microfluidic chip liquid chromatography and tandem mass spectrometry.

@article{Nwosu2012ComparisonOT,
  title={Comparison of the human and bovine milk N-glycome via high-performance microfluidic chip liquid chromatography and tandem mass spectrometry.},
  author={Charles C Nwosu and Danielle L Aldredge and Hyeyoung Lee and Larry A Lerno and Angela M. Zivkovic and J. Bruce German and Carlito B. Lebrilla},
  journal={Journal of proteome research},
  year={2012},
  volume={11 5},
  pages={2912-24}
}
The isolation of whey proteins from human and bovine milks followed by profiling of their entire N-glycan repertoire is described. Whey proteins resulting from centrifugation and ethanol precipitation of milk were treated with PNGase F to release protein-bound N-glycans. Once released, N-glycans were analyzed via nanoflow liquid chromatography coupled with… CONTINUE READING