Comparison of the established standard complement-dependent cytotoxicity and flow cytometric crossmatch assays with a novel ELISA-based HLA crossmatch procedure.

@article{Altermann2006ComparisonOT,
  title={Comparison of the established standard complement-dependent cytotoxicity and flow cytometric crossmatch assays with a novel ELISA-based HLA crossmatch procedure.},
  author={Wolfgang W. Altermann and Barbara Seliger and Saadettin Sel and Dan J. Wendt and Gerald Schlaf},
  journal={Histology and histopathology},
  year={2006},
  volume={21 10},
  pages={1115-24}
}
The detection of donor-specific anti-HLA antibodies by standard procedures such as complement-dependent cytotoxicity assay (CDC) or flow cytometric (FACS) analysis is limited by its low sensitivity and the quality of the donor cells. Therefore, an ELISA-based technique was employed using solid phase-immobilized monoclonal antibodies to capture HLA class I… CONTINUE READING