Comparison of the effect of mitochondrial inhibitors on mitochondrial membrane potential in two different cell lines using flow cytometry and spectrofluorometry.

@article{Kalbacova2003ComparisonOT,
  title={Comparison of the effect of mitochondrial inhibitors on mitochondrial membrane potential in two different cell lines using flow cytometry and spectrofluorometry.},
  author={Marie Kalbacova and Marek Vrback{\'y} and Zdeněk Drahota and Zora Mělkov{\'a}},
  journal={Cytometry. Part A : the journal of the International Society for Analytical Cytology},
  year={2003},
  volume={52 2},
  pages={110-6}
}
BACKGROUND Determination of mitochondrial membrane potential (DeltaPsim) is widely used to characterize cellular metabolism, viability, and apoptosis. Changes of DeltaPsim induced by inhibitors of oxidative phosphorylation characterize respective contributions of mitochondria and glycolysis to adenosine triphosphate (ATP) synthesis. METHODS DeltaPsim in BSC-40 and HeLa G cell lines was determined by flow cytometry and spectrofluorometry. Its changes induced by specific mitochondrial… CONTINUE READING

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