Comparison of the Pasteurella haemolytica A1 envelope proteins obtained by two cell disruption methods.

@article{Simons1989ComparisonOT,
  title={Comparison of the Pasteurella haemolytica A1 envelope proteins obtained by two cell disruption methods.},
  author={Kellie Simons and Rebecca J. Morton and Derek A Mosier and Robert W. Fulton and Anthony W. Confer},
  journal={Journal of clinical microbiology},
  year={1989},
  volume={27 4},
  pages={664-7}
}
The French pressure cell and sonication methods of bacterial cell disruption were compared for the evaluation of surface proteins from Pasteurella haemolytica A1. Several protein bands were quantitatively different when compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and densitometry. With densitometry, sonicated preparations had higher concentrations of the 102-, 83-, 50-, 38-, and 30-kilodalton (kDa) proteins; French pressure cell preparations had higher… CONTINUE READING