Comparison of the LDH and MTT assays for quantifying cell death: validity for neuronal apoptosis?

@article{Lobner2000ComparisonOT,
  title={Comparison of the LDH and MTT assays for quantifying cell death: validity for neuronal apoptosis?},
  author={Doug Lobner},
  journal={Journal of neuroscience methods},
  year={2000},
  volume={96 2},
  pages={147-52}
}
  • Doug Lobner
  • Published 2000 in Journal of neuroscience methods
Neuronal apoptosis induced in cortical cultures by exposure to serum deprivation, staurosporine, nifedipine, or C2-ceramide was assayed by lactate dehydrogenase (LDH) release or inhibition of 3-(4, 5-dimethylthiazol-2-yl)2,5-diphenyl-tetrazolium bromide (MTT) reduction. The protective effects of neurotrophin-4, Z-Val-Ala-Asp-fluoromethylketone (ZVAD), and… CONTINUE READING