Comparison of the 5′ and 3′ termini of tomato ringspot virus RNA1 and RNA2: Evidence for RNA recombination☆

@article{Rott1991ComparisonOT,
  title={Comparison of the 5′ and 3′ termini of tomato ringspot virus RNA1 and RNA2: Evidence for RNA recombination☆},
  author={Michael E Rott and Jack H. Tremaine and D. M. Rochon},
  journal={Virology},
  year={1991},
  volume={185},
  pages={468 - 472}
}
Abstract The sequences of the 5′ terminal 1140 and 3′ terminal 1546 nt of tomato ringspot virus (TomRSV) RNA1 have been determined. These sequences share a high degree of nucleotide sequence similarity with the previously determined TomRSV RNA2 sequence. Eighty-eight percent of the 5' terminal 907 nt of TomRSV RNA1 and RNA2 contain identical nucleotide residues; the first 459 nt are identical at all positions, whereas the next 447 nt are identical at only 75.8% of the nucleotide positions… 
Sequence analysis of the 3' termini of RNA1 and RNA2 of blueberry leaf mottle virus
Abstract The 3' termini of RNA1 and RNA2 of blueberry leaf mottle virus (BBLMV) were cloned and the cDNA sequence of a portion of the putative polymerase gene, the complete coat protein (CP)
Nucleotide sequence of tomato ringspot virus RNA-2.
TLDR
Comparisons of the coding and non-coding regions of TomRSV RNA-2 and the RNA components of TBRV, GCMV, GFLV and the comovirus cowpea mosaic virus revealed significant similarity for over 300 amino acids between the coding region immediately to the N-terminal side of the putative coat proteins ofTomRSV and GFLv.
Nucleotide sequence of tomato ringspot virus RNA1.
TLDR
The nucleotide sequence of tomato ringspot nepovirus (TomRSV) RNA1 has been determined and a conserved histidine residue present in the proteases encoded by members of the como-, poty- and poliovirus groups which is thought to be involved in dipeptide cleavage site recognition is found.
Mapping of sequences in the 5’ region and 3’ UTR of tomato ringspot virus RNA2 that facilitate cap-independent translation of reporter transcripts in vitro
TLDR
The general mechanism of ToRSV RNA2 translation initiation is similar to that previously reported for the RNAs of blackcurrant reversion virus, another nepovirus, however, the position, sequence and predicted structures of the translation-enhancing elements differed between the two viruses.
Characterization of the partial RNA1 and RNA2 3′ untranslated region of Tomato ringspot virus isolates from North America
TLDR
RT-PCR using primers designed within the highly conserved 3′ UTR regions detected all 18 ToRSV isolates as well as two isolates from a vineyard, suggesting this assay can serve as a practical methodology for the sensitive detection of varied To RSV isolate as it is more sensitive than a RT-PCRs based on previously reported U1/D1 primers.
Cloning and sequencing of peach rosette mosaic virus RNA1.
TLDR
Analysis of PRMV RNA1 further distinguishes the taxonomic subdivisions within the nepovirus group, confirms the subgroup three status ofPRMV and lays the groundwork for a replicase-mediated resistance strategy.
Complete Nucleotide Sequence and Infectious cDNA Clone of the RNA1 of a Chinese Isolate of Broad Bean Wilt Virus 2
TLDR
Amino acid sequence comparisons between portions of the BBWV2 RNA1-encoded polyprotein and proteins encoded by several species in Comoviridae revealed the putative functions of BBW V2 RNA 1-encoding proteins and the same general genetic organization as that of comoviruses and nepovIRuses.
Long, nearly identical untranslated sequences at the 3′ terminal regions of the genomic RNAs of cherry leafroll virus (walnut strain)
Hybridization analyses of cDNA clones derived from the two genomic RNAs, RNA1 and RNA2, of the walnut strain of the nepovirus cherry leafroll nepovirus (wCLRV) demonstrated a long region of high
The nucleotide sequence of the RNA-2 of an isolate of the English serotype of tomato black ring virus: RNA recombination in the history of nepoviruses.
TLDR
Sequence comparisons indicate that TBRV-ED RNA-2 probably arose by an RNA recombination event that resulted in the exchange of the putative movement protein gene between T BRV and GCMV.
Nucleotide sequence of black currant reversion associated nepovirus RNA1.
TLDR
Con Conserved amino acid motifs, characteristic for the viral protease co-factor, the NTP-binding protein, the cysteine protease and the RdRp core domains, known to occur in the polyproteins of different viruses of the picornavirus-like supergroup, are all detected in the amino acid sequences encoded by BRAV RNA1.
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TLDR
Southern blot analyses using clones derived from RNA-1 and RNA-2 confirmed that there is a 1.9 kb nucleotide sequence homology at the 3′ termini of tomato ringspot virus, which has not been reported previously for other plant viruses with multipartite ssRNA genomes.
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