Comparison of strong cation exchange and SDS-PAGE fractionation for analysis of multiprotein complexes.

@article{Das2010ComparisonOS,
  title={Comparison of strong cation exchange and SDS-PAGE fractionation for analysis of multiprotein complexes.},
  author={Sudipto Das and Allen D. Bosley and Xiaoying Ye and King Chung Chan and Isabel W. T. Chu and Jeffery E. Green and Haleem J. Issaq and Timothy D. Veenstra and Thorkell Andr{\'e}sson},
  journal={Journal of proteome research},
  year={2010},
  volume={9 12},
  pages={6696-704}
}
Affinity purification of protein complexes followed by identification using liquid chromatography/mass spectrometry (LC-MS/MS) is a robust method to study the fundamental process of protein interaction. Although affinity isolation reduces the complexity of the sample, fractionation prior to LC-MS/MS analysis is still necessary to maximize protein coverage… CONTINUE READING