Comparison of single regulated lentiviral vectors with rtTA expression driven by an autoregulatory loop or a constitutive promoter

@article{Markusic2005ComparisonOS,
  title={Comparison of single regulated lentiviral vectors with rtTA expression driven by an autoregulatory loop or a constitutive promoter},
  author={David M Markusic and Ronald P. J. Oude-Elferink and Atze T. Das and Ben Berkhout and Jurgen Seppen},
  journal={Nucleic Acids Research},
  year={2005},
  volume={33},
  pages={e63 - e63}
}
Regulated expression of a therapeutic gene is crucial for safe and efficacious gene therapy. Many inducible regulatory systems use a constitutive promoter to express a regulatory protein, such as rtTA in the Tet-On system, which may restrict their use because of cytotoxicity and immunogenicity. Autoregulatory expression of rtTA provides extremely low levels of rtTA when transgene expression is off, with rapid transgene induction upon addition of doxycycline. Lentiviral vectors efficiently… CONTINUE READING

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