Comparison of separation modes of high-performance liquid chromatography for the analysis of glycoprotein- and proteoglycan-derived oligosaccharides.

Abstract

Porous graphitised carbon (PGC) has been explored for high-performance liquid chromatography (HPLC) of mono- and di-saccharides released from proteoglycans and of fluorescently labelled oligosaccharide derivatives for high-sensitivity detection. Sulphated oligosaccharides show good retention and separation behaviour on PGC-HPLC, and compared to anion-exchange or reversed-phase ion-pair chromatography the chromatography is carried out in the absence of salt. Due to their poor retention on PGC-HPLC the analysis of single uronic acids has been optimised with high pH anion-exchange chromatography. Fluorescent labelled derivatives formed by reductive amination of neutral oligosaccharides with 2-aminobenzamide have been chromatographed on PGC-HPLC and by BioGel P4 gel filtration.

Cite this paper

@article{Davies1996ComparisonOS, title={Comparison of separation modes of high-performance liquid chromatography for the analysis of glycoprotein- and proteoglycan-derived oligosaccharides.}, author={Melanie J Davies and Elizabeth F. Hounsell}, journal={Journal of chromatography. A}, year={1996}, volume={720 1-2}, pages={227-33} }