Comparison of restriction endonuclease analysis and phenotypic typing methods for differentiation of Yersinia enterocolitica isolates.

@article{Kapperud1990ComparisonOR,
  title={Comparison of restriction endonuclease analysis and phenotypic typing methods for differentiation of Yersinia enterocolitica isolates.},
  author={Georg Kapperud and Truls Nesbakken and Svetlana Aleksi{\'c} and Henri H. Mollaret},
  journal={Journal of clinical microbiology},
  year={1990},
  volume={28 6},
  pages={1125-31}
}
Restriction endonuclease analysis of chromosomal DNA (REAC) was used to study polymorphism in restriction fragment patterns among Yersinia enterocolitica isolates belonging to serogroups O3, O5,27, O8, O9, O13, and O21. Using the enzyme HaeIII and electrophoresis on thin (0.75-mm) vertical 5% polyacrylamide gels, we were able to distinguish at least 22 DNA fragment patterns among the 72 strains examined. The method showed the greatest discriminatory power with regard to serogroup O8, within… CONTINUE READING