Comparison of recombinant human PDE4 isoforms: interaction with substrate and inhibitors.

  title={Comparison of recombinant human PDE4 isoforms: interaction with substrate and inhibitors.},
  author={Natalie Saldou and Rena Obernolte and Anton S. Huber and Preston A. Baecker and Ren{\'e} Wilhelm and Robert Alvarez and Baojie Li and Li-ming Xia and Ondine Callan and Chao Su and Kurt Jarnagin and Earl R. Shelton},
  journal={Cellular signalling},
  volume={10 6},
Four cyclic-nucleotide phosphodiesterase (PDE) genes belonging to the PDE4 family (PDE4A, 4B, 4C and 4D) have been identified. All four isogenes, including several deletions and alterations of the amino, carboxyl and central catalytic domains, were expressed in insect cells. Lysates were characterised for enzyme activity by using the Km for substrate and the EC50 for activation by the cofactor Mg2+. The catalytic domain alone appears to be sufficient for the normal enzymatic function of PDE4… CONTINUE READING


Publications citing this paper.
Showing 1-10 of 16 extracted citations

Small molecule allosteric modulators of phosphodiesterase 4.

Handbook of experimental pharmacology • 2011
View 4 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…