Comparison of real-time reverse transcriptase polymerase chain reaction of peripheral blood mononuclear cells, serum and cell-free body cavity effusion for the diagnosis of feline infectious peritonitis

@article{Doenges2017ComparisonOR,
  title={Comparison of real-time reverse transcriptase polymerase chain reaction of peripheral blood mononuclear cells, serum and cell-free body cavity effusion for the diagnosis of feline infectious peritonitis},
  author={Stephanie J Doenges and K. Weber and R. Dorsch and R. Fux and K. Hartmann},
  journal={Journal of Feline Medicine and Surgery},
  year={2017},
  volume={19},
  pages={344 - 350}
}
Objectives Diagnosis of feline infectious peritonitis (FIP) remains challenging, especially in cats without effusions. The objective of this study was to evaluate the sensitivity and specificity of a real-time reverse transcriptase polymerase chain reaction (RT-PCR) detecting feline coronavirus (FCoV) RNA in peripheral blood mononuclear cells (PBMCs) and serum in comparison with the same real-time RT-PCR in cell-free body cavity effusion. Methods This prospective case-control study included 92… Expand
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Although the RT-LAMP assay is less sensitive than real time reverse transcription PCR (RT-PCR), it can be performed without the need of expensive equipment and with less hands-on time, and might lead to a suitable in-house test and accelerate the diagnosis of FIP. Expand
Detection of feline coronavirus RNA, spike gene mutations, and feline coronavirus antigen in macrophages in aqueous humor of cats in the diagnosis of feline infectious peritonitis
  • Laura Sangl, S. Felten, +6 authors K. Hartmann
  • Medicine
  • Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
  • 2020
TLDR
The combination of 7b- RT-rtPCR and IHC could be useful in diagnosing FIP; S-RT-RTPCR did not add value; and ICC of aqueous humor samples cannot be recommended for the diagnosis of FIP. Expand
Feline coronavirus with and without spike gene mutations detected by real-time RT-PCRs in cats with feline infectious peritonitis
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In these 20 cats with FIP, FCoVs with S gene mutations were found in every cat in at least one tissue or fluid sample, which highlights the association between mutated S gene and systemic FCoV spread. Expand
Detection of feline coronavirus mutations in paraffin-embedded tissues in cats with feline infectious peritonitis and controls
TLDR
FCoV was detected in low concentration in tissues of control animals, confirming the ability of FCoV to spread systemically and underestimating the sensitivity of the RT-qPCR. Expand
Limitations of using feline coronavirus spike protein gene mutations to diagnose feline infectious peritonitis
TLDR
It is demonstrated that S gene mutation analysis in FCoVs does not substantially improve the ability to diagnose FIP as compared to detection of FCoV alone. Expand
Immunocytochemistry of mesenteric lymph node fine-needle aspirates in the diagnosis of feline infectious peritonitis
TLDR
Given that a false-positive result occurred and FIP was correctly detected in only approximately half of the cases of FIP, ICC of mesenteric lymph node FNA alone cannot reliably confirm or exclude FIP but can be a helpful test in conjunction with other diagnostic measures. Expand
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