Comparison of real-time PCR methods for detection of Salmonella enterica and Escherichia coli O157:H7, and introduction of a general internal amplification control.

@article{Klerks2004ComparisonOR,
  title={Comparison of real-time PCR methods for detection of Salmonella enterica and Escherichia coli O157:H7, and introduction of a general internal amplification control.},
  author={Michel M Klerks and Carolien Zijlstra and Ariena H. C. van Bruggen},
  journal={Journal of microbiological methods},
  year={2004},
  volume={59 3},
  pages={337-49}
}
The objectives of this study were to compare different real-time PCR-based methods for detection of either Salmonella spp. or E. coli O157:H7 with respect to sensitivity, precision and accuracy. In addition, a general internal amplification control (IAC) is presented, allowing prevention of false negative results. The IAC allows insight in amplification efficiency and enables a more accurate quantification with the evaluated real-time PCR methods. Implementation of the IAC with the different… CONTINUE READING

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