Comparison of rapid quantitative PCR-based and conventional culture-based methods for enumeration of Enterococcus spp. and Escherichia coli in recreational waters.

@article{Noble2010ComparisonOR,
  title={Comparison of rapid quantitative PCR-based and conventional culture-based methods for enumeration of Enterococcus spp. and Escherichia coli in recreational waters.},
  author={Rachel T Noble and Angelia D Blackwood and John F. Griffith and Charles D. McGee and Stephen B Weisberg},
  journal={Applied and environmental microbiology},
  year={2010},
  volume={76 22},
  pages={7437-43}
}
Recreational water quality is currently monitored using culture-based methods that require 18 to 96 h for results. Quantitative PCR (QPCR) methods that can be completed in less than 2 h have been developed, but they could yield different results than the conventional methods. We present two studies in which samples were processed simultaneously for Enterococcus spp. and Escherichia coli using two culture-based methods (EPA method 1600 and Enterolert/Colilert-18) and QPCR. The proprietary QPCR… CONTINUE READING

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