Comparison of rapid DNA extraction methods applied to PCR identification of medicinal mushroom Ganoderma spp.

@article{Zhou2007ComparisonOR,
  title={Comparison of rapid DNA extraction methods applied to PCR identification of medicinal mushroom Ganoderma spp.},
  author={Xuanwei Zhou and Qizhang Li and Jingya Zhao and Kexuan Tang and J. -L. Lin and Yizhou Yin},
  journal={Preparative biochemistry & biotechnology},
  year={2007},
  volume={37 4},
  pages={369-80}
}
Four different DNA extraction methods were used to extract genomic DNA of the medicinal mushroom Lingzhi from its developing stage materials, such as mycelium, dry fruiting body, or sliced and spore powder or sporoderm-broken spore powder. The DNA samples were analyzed using agarose gel electrophoresis, UV spectrophotometer, and PCR amplification. According to the average yields and purity of DNA, high salt concentrations and low pH methods were the best for DNA extraction. The mycelia and… CONTINUE READING