Comparison of radio-iodinated serum albumin and blue dextran as indicators to measure rate of formation of cerebrospinal fluid

  title={Comparison of radio-iodinated serum albumin and blue dextran as indicators to measure rate of formation of cerebrospinal fluid},
  author={A. Martins and A. Ramirez and T. Doyle},
  journal={Experimental Neurology},
Abstract The characteristics of two nondiffusible indicators, 125I-labeled albumin (RISA-125) and blue dextran, were compared by using them simultaneously to measure rate of formation of cerebrospinal fluid (CSF) in in vitro experiments and in a series of ventriculocisternal perfusion experiments in rhesus monkeys. Mean CSF formation calculated from the dilution of RISA-125 was 37.6 μl/min (±0.74 sem ); the CSF formation calculated from the corresponding dilution of blue dextran was 37.1 μl/min… 
Estimation of lung liquid production in fetal sheep with blue dye dextran and radioiodinated serum albumin.
The data suggest that there was no significant difference in production or reabsorption rates measured by BDD or RISA, differences in volumes measured by the two indicators were only significant if gestational age was ignored and were too small to have physiological importance, and although BDD and RISA each may have methodological weaknesses, for purposes of measuring lung liquid volumes both are sufficiently accurate and reproducible to obtain meaningful physiological results.
Sources of error in measuring cerebrospinal fluid formation by ventriculocisternal perfusion.
It is concluded that to assess accurately the effect a variable has on the rate of CSF formation, one must control perfusion time and craniospinal blood volume as well as intracranial pressure.
Saturable transport of cimetidine from cerebrospinal fluid to blood in rats.
It is suggested that cimetidine is excreted from CSF to blood by a saturable transport system.
A chronic sheep preparation for the study of drug pharmacokinetics in spinal and ventricular CSF.
The simultaneous appearance of morphine and [14C]sucrose in cisternal CSF after lumbar subarachnoid administration indicates that morphine, like sucrose, is distributed within the CSF by bulk flow.
Effects of hypercapnia and papaverine on cerebrospinal fluid formation and choroid plexus blood flow.
The choroid plexus, a major source of CSF, is demonstrated to be less reactive to vasodilatation induced by either hypercapnia or a vasoactive agent, and favorably explanes, at least partially, the unchanged Vf under the effect of hyper capnia or papaverine.
ventriculocisternal perfusion . cerebrospinal fluid formation by Sources of error in measuring
error. Using the technique to measure CSF formation rate in the rhesus monkey, we have observed rate changes when none were expected. Most puzzling has been the steady decline of CSF formation rate
Entry of neutralizing antibody to measles into brain and cerebrospinal fluid of immunized monkeys after osmotic opening of the blood-brain barrier
Brain antibody remained elevated for at least 4 days following osmotic opening, and cerebrospinal fluid concentrations were tenfold higher during this period, both for grade 2+ and 3+ openings, suggesting that tight junctions between cerebrovascular endothelia were widened to molecules with a molecular weight of at least 180,000.
Lung liquid production by in vitro lungs from fetal guinea pigs: studies with metabolic inhibitors.
The results suggest that lung liquid production requires glycolysis and aerobic metabolism, however, reabsorption appears to continue on gly colysis alone, a particularly useful situation for neonates suffering respiratory distress.
The circadian variation of catecholamine metabolism in the subhuman primate
Cerebrospinal fluid (CSF) was removed continuously in 2- or 3-h aliquots from the lateral and fourth cerebral ventricles of chronic chair restrained rhesus monkeys. Under conditions of 12 h light
Cerebrospinal Fluid β-Amyloid Turnover in the Mouse, Dog, Monkey and Human Evaluated by Systematic Quantitative Analyses
The turnover of CSF Aβ40 was systematically examined, for the first time, in multiple species through quantitative modeling of multiple data sets, and the result suggests that the clearance mechanisms for CSf Aβ in rodents may be different from those in the higher species.


Cerebrospinal fluid production rates determined by simultaneous albumin and inulin perfusion.
Brain tissue slices from the paraventricular regions of the lateral, third, and fourth ventricles demonstrated an uptake of 14C-inulin two to five times greater than that of albumin, indicating that inulin is lost from the ventriculocisternal system to a greater extent than has been previously recognized.
The effect of anaesthetic agents on the cerebrospinal fluid clearance of 35S-sulphate and 125-I-iodide.
It is suggested that the reduced CSF clearance observed in vivo was a result, at least in part, of inhibition of choroid plexus transport, which is commonly used in the laboratory on the clearance of 35S-sulfate and 125I-iodide.
Effect of certain drugs on cerebrospinal fluid production in the dog
Cerebrospinal fluid (CSF) production rates were measured in the dog by ventriculocisternal perfusion with inulin containing buffer. Normal CSF production rate was found to be about 0.05 ml/min which
Origin of cerebrospinal fluid gamma globulin in subacute sclerosing leukoencephalitis.
Results were obtained which suggest that immunoglobulin is synthesized within the nervous system in SSLE, which is generally fatal in subacute sclerosing leukoencephalitis.
Overproduction of cerebrospinal fluid in communicating hydrocephalus
A markedly excessive rate of CSF formation was found during steady-state ventriculolumbar perfusion, suffocating a child with communicating hydrocephalus in whom a choroid plexus papilloma was not felt to be present.
The production of cerebrospinal fluid in man and its modification by acetazolamide.
The availability at the National institutes of Heal th of a group of patients undergoing cerebrospinal fluid perfusion chemotherapy for the treatment of central nervous system neoplasm afforded the opportunity for the opportunity to study the effects of acetazolamide on spinal fluid production and absorption.
Cerebrospinal Fluid Production by the Choroid Plexus and Brain
In both normal and plexectomized animals, 24Na levels were found to be markedly greater in the gray matter surrounding the ventricles and in thegray matter bordering the subarachnoid space, suggesting that sodium exchanges in these two general areas of the brain may be linked to the formation of the cerebrospinal fluid.
Extracellular space of brain as determined by diffusion of inulin from the ventricular system
Abstract Perfusion of the ventriculo-cisternal system of the live dog with artificial spinal fluid containing radioactive inulin, sulfate or dextran resulted in the entry of these compounds into the
Effects of Acid-Base Alterations on Cerebrospinal Fluid Production
  • W. Oppelt, T. Maren, E. Owens, D. Rall
  • Chemistry, Medicine
    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine
  • 1963
The addition of acetazolamide during metabolic acidosis and alkalosis as well as during respiratory alkalotic treatment caused a significant decrease in CSF production rates as compared to production during the periods of acid-base alterations alone.
The effects of some inhibitors and accelerators of sodium transport on the turnover of 22Na in the cerebrospinal fluid and the brain
The purpose of the experiments was to discover whether the turnover of 22Na in the c.s.f. is affected in the same manner by inhibitors or accelerators of active transport as the turnover in the brain tissue since there is reason to believe that the composition of the extracellular fluid of brain is controlled by active processes.