Comparison of quenching and extraction methodologies for metabolome analysis of Lactobacillus plantarum

@article{Faijes2007ComparisonOQ,
  title={Comparison of quenching and extraction methodologies for metabolome analysis of Lactobacillus plantarum},
  author={Magda Faijes and Astrid Elisabeth Mars and Eddy J. Smid},
  journal={Microbial Cell Factories},
  year={2007},
  volume={6},
  pages={27 - 27}
}
BackgroundA reliable quenching and metabolite extraction method has been developed for Lactobacillus plantarum. The energy charge value was used as a critical indicator for fixation of metabolism.ResultsFour different aqueous quenching solutions, all containing 60% of methanol, were compared for their efficiency. Only the solutions containing either 70 mM HEPES or 0.85% (w/v) ammonium carbonate (pH 5.5) caused less than 10% cell leakage and the energy charge of the quenched cells was high… CONTINUE READING
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