Comparison of protocols for measuring activities of human blood cholinesterases by the Ellman method.

@article{Reiner2000ComparisonOP,
  title={Comparison of protocols for measuring activities of human blood cholinesterases by the Ellman method.},
  author={Elsa Reiner and Goran {\vS}inko and Mira {\vS}krinjari{\'c}-{\vS}poljar and V Simeon-Rudolf},
  journal={Arhiv za higijenu rada i toksikologiju},
  year={2000},
  volume={51 1},
  pages={13-8}
}
This paper presents a protocol for routine assays of human blood cholinesterase activities which separates erythrocytes from plasma by centrifugation and measures acetylcholinesterase activity in unwashed erythrocytes and butyrylcholinesterase activity in the plasma. The recommended substrate for both enzymes is 1.0 mM acetylthiocholine. The protocol is compared with other two recommended protocols for the activity measurements of the two enzymes using the Ellman method. The paper discusses the… CONTINUE READING