Comparison of predictive methods and biological validation for qPCR reference genes in sunflower leaf senescence transcript analysis

@article{Fernandez2010ComparisonOP,
  title={Comparison of predictive methods and biological validation for qPCR reference genes in sunflower leaf senescence transcript analysis},
  author={Paula Fernandez and Julio A. Di Rienzo and Sebasti{\'a}n Moschen and Guillermo A. A. Dosio and Luis A. N. Aguirrez{\'a}bal and Horacio Esteban Hopp and Norma Beatriz Paniego and Ruth A. Heinz},
  journal={Plant Cell Reports},
  year={2010},
  volume={30},
  pages={63-74}
}
The selection and validation of reference genes constitute a key point for gene expression analysis based on qPCR, requiring efficient normalization approaches. In this work, the expression profiles of eight genes were evaluated to identify novel reference genes for transcriptional studies associated to the senescence process in sunflower. Three alternative strategies were applied for the evaluation of gene expression stability in leaves of different ages and exposed to different treatments… CONTINUE READING