Comparison of phase contrast transmission electron microscopy with optimized scanning transmission annular dark field imaging for protein imaging.

@article{Rez2003ComparisonOP,
  title={Comparison of phase contrast transmission electron microscopy with optimized scanning transmission annular dark field imaging for protein imaging.},
  author={Peter Rez},
  journal={Ultramicroscopy},
  year={2003},
  volume={96 1},
  pages={117-24}
}
Henderson has already shown that electron microscopy should be superior to X-ray and neutron diffraction for determining protein structure with minimum radiation damage. Since the contrast for a molecule embedded in vitreous ice is very low, it is conceivable that dark field imaging would be superior to bright field phase contrast microscopy. A detailed analysis of contrast and signal/noise for both imaging modes is presented. Annular dark field scanning transmission microscopy gives improved… CONTINUE READING

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