Comparison of partial SSUrDNA sequences suggests revisions of species names in the genus Naegleria

  title={Comparison of partial SSUrDNA sequences suggests revisions of species names in the genus Naegleria},
  author={Johan F. de Jonckheere},
  journal={European Journal of Protistology},
  • J. Jonckheere
  • Published 29 August 1994
  • Biology
  • European Journal of Protistology
Fish-isolated Naegleria strains and their phylogeny inferred from ITS and SSU rDNA sequences.
Evidence of a need to re-evaluate the current practice of setting boundaries between species of the genus Naegleria is provided, as phylogenetic analyses of separate and concatenated SSU rDNA and ITS sequences revealed phylogenetic relationships of strains under study.
Three different group I introns in the nuclear large subunit ribosomal DNA of the amoeboflagellate Naegleria.
We have amplified the large subunit ribosomal DNA (LSUrDNA) of the 12 described Naegleria spp. and of 34 other Naegleria lineages that might be distinct species. Two strains yielded a product that is
The amoeba-to-flagellate transformation test is not reliable for the diagnosis of the genus Naegleria. Description of three new Naegleria spp.
Failing to form flagellates since their isolation, even when different transformation procedures are used, are two Naegleria strains from Chile and Indonesia, which are proposed to represent new species.
Analysis of the 5.8S rRNA Gene and the Internal Transcribed Spacers in Naegleria spp. and in N. fowleri
The results showed that this region can help differentiate between and within species, and it was possible to define species specific primers in ITS regions to rapidly identify N. fowleri.
Identity of Naegleria strains isolated from organs of freshwater fishes.
Eighteen Naegleria strains were isolated from organs of freshwater fishes belonging to 5 species and, based on the SSU rRNA sequences and riboprints, RFLP-PCR patterns appeared in phylogenetic trees to be closely related to Naeglersia clarki.


A Group I Intron in the SSUrDNA of Some Naegleria spp. Demonstrated by Polymerase Chain Reaction Amplification
ABSTRACT. The small subunit ribosomal DNA (SSUrDNA) of all described Naegleria spp. was amplified by polymerase chain reaction with universal primers. In all strains of N. andersoni andersoni, N.
Discontinuous genetic variation among mesophilic Naegleria isolates: further evidence that N. gruberi is not a single species.
Characteristics temperature limits for growth show that the mesophilic species are ecological as well as genetic entities, most consistent with the interpretation that N. gruberi is a group of several distinct species.
srRNA evolution and phylogenetic relationships of the genus Naegleria (Protista: Rhizopoda).
It was shown that the semiconserved regions of the srRNA molecule evolve in a clocklike fashion and that the clock is time dependent rather than generation dependent.
A genetic approach to species criteria in the amoeba genus Naegleria using allozyme electrophoresis.
Large genetic heterogeneity within amoebas of the species Naegleria gruberi and evolutionary affinities to the other species of the genus.
The allozyme survey was extended to 7 strains of Naegleria gruberi and N. jadini in order to further characterize the genetic structure of these free-living amoebas. As formerly known for several
Phylogenetic evidence for the acquisition of ribosomal RNA introns subsequent to the divergence of some of the major Tetrahymena groups.
Complete small subunit ribosomal RNA gene sequences from 13 species of Tetrahymena are determined and the absolute number of nucleotide differences between the sequences was used to construct a phylogenetic tree to indicate that the intron character has been acquired independently in different species at a stage later than the branching out of the species.
A comparative study of 14 strains of Naegleria australiensis demonstrates the existence of a highly virulent subspecies: N. australiensis italica n. spp.
Naegleria australiensis does not appear to be as homogenous a species as N. fowleri and seems to be more widespread than previously thought.