Comparison of oocyte-activating agents for mouse cloning.

  title={Comparison of oocyte-activating agents for mouse cloning.},
  author={Hidefumi Kishikawa and Teruhiko Wakayama and Ryuzo Yanagimachi},
  volume={1 3},
Since somatic cell components are unable to activate oocytes following injection or fusion, enucleated oocytes receiving adult somatic cells during the cloning process must be activated artificially for their development. We compared the efficiency of four types of oocyte-activating agents: strontium, ethanol, single electric pulse, and spermatozoa. Although strontium was the best in supporting preimplantation development of reconstructed mouse oocytes, there was no significant difference among… CONTINUE READING

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