Comparison of one-dimensional and two-dimensional gel electrophoresis as a separation tool for proteomic analysis of rat liver microsomes: cytochromes P450 and other membrane proteins.

@article{Galeva2002ComparisonOO,
  title={Comparison of one-dimensional and two-dimensional gel electrophoresis as a separation tool for proteomic analysis of rat liver microsomes: cytochromes P450 and other membrane proteins.},
  author={Nadezhda A. Galeva and Michail Altermann},
  journal={Proteomics},
  year={2002},
  volume={2 6},
  pages={713-22}
}
Gel electrophoresis in combination with peptide mass fingerprinting is the method of choice for proteomic profiling of various in vitro and in vivo biological systems. In the investigation reported here we analyzed the protein composition of hepatic microsomes from untreated and phenobarbital treated rats, using one-dimensional (1-DE) and two-dimensional (2-DE) gel electrophoresis, followed by tryptic peptide mapping. To better characterize capabilities of 2-DE 1-DE with regard to microsomal… CONTINUE READING

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