Comparison of nucleic acid-protein interactions in solution and in polyacrylamide gels.


The properties of nucleic acid-protein complexes in polyacrylamide gels and in solution have been compared. Transcription by RNA polymerase from a lac DNA template within a gel yields products which are the same as those obtained in solution. Likewise the dissociation rates of DNA-protein complexes in a gel are found to be identical to the solution values… (More)