Comparison of normalization methods for construction of large, multiplex amplicon pools for next-generation sequencing.

@article{Harris2010ComparisonON,
  title={Comparison of normalization methods for construction of large, multiplex amplicon pools for next-generation sequencing.},
  author={J. Kirk Harris and Jason W. Sahl and Todd A. Castoe and Brandie D. Wagner and David D. Pollock and John R Spear},
  journal={Applied and environmental microbiology},
  year={2010},
  volume={76 12},
  pages={3863-8}
}
Constructing mixtures of tagged or bar-coded DNAs for sequencing is an important requirement for the efficient use of next-generation sequencers in applications where limited sequence data are required per sample. There are many applications in which next-generation sequencing can be used effectively to sequence large mixed samples; an example is the characterization of microbial communities where <or=1,000 sequences per samples are adequate to address research questions. Thus, it is possible… CONTINUE READING

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