Comparison of nested polymerase chain reaction (PCR), real-time PCR and viral culture for the detection of cytomegalovirus in subgingival samples.

@article{Botero2008ComparisonON,
  title={Comparison of nested polymerase chain reaction (PCR), real-time PCR and viral culture for the detection of cytomegalovirus in subgingival samples.},
  author={Javier Enrique Botero and Carmen Vidal and Adolfo V{\'a}zquez Contreras and Beatriz Elena Parra},
  journal={Oral microbiology and immunology},
  year={2008},
  volume={23 3},
  pages={239-44}
}
INTRODUCTION The purpose of this study was to compare nested polymerase chain reaction (PCR), real-time PCR, and shell vial for the detection of human cytomegalovirus (HCMV) in subgingival samples in periodontitis patients. METHODS A group of 44 patients and 24 individuals without periodontitis were included in the study. A full periodontal examination was conducted in each subject. Gingival crevicular fluid (GCF) was collected by pocket lavage and used for viral culture (shell vial… CONTINUE READING

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