Comparison of multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) for genetic typing of Staphylococcus aureus.

Abstract

We compared multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) for typing of Staphylococcus aureus and show that the methods yield similar results, although with differences in resolving power and reproducibility. Epidemiological conditions should determine which is the optimal typing method to be used.

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@article{Melles2007ComparisonOM, title={Comparison of multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) for genetic typing of Staphylococcus aureus.}, author={Damian C. Melles and Willem B. van Leeuwen and Susan V. Snijders and Deborah Horst-Kreft and Justine K. Peeters and Henri A. Verbrugh and Alex van Belkum}, journal={Journal of microbiological methods}, year={2007}, volume={69 2}, pages={371-5} }