Comparison of methods for glycogen analysis of in vitro fermentation pellets produced with strained ruminal inoculum.

@article{Hall2015ComparisonOM,
  title={Comparison of methods for glycogen analysis of in vitro fermentation pellets produced with strained ruminal inoculum.},
  author={Mary Beth Hall and Ronald D. Hatfield},
  journal={Journal of microbiological methods},
  year={2015},
  volume={118},
  pages={
          147-51
        }
}
Microbial glycogen measurement is used to account for fates of carbohydrate substrates. It is commonly applied to washed cells or pure cultures which can be accurately subsampled, allowing the use of smaller sample sizes. However, the nonhomogeneous fermentation pellets produced with strained rumen inoculum cannot be accurately subsampled, requiring analysis of the entire pellet. In this study, two microbial glycogen methods were compared for analysis of such fermentation pellets: boiling… CONTINUE READING
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