Comparison of methods for extracting thylakoid membranes of Arabidopsis plants.

@article{Chen2016ComparisonOM,
  title={Comparison of methods for extracting thylakoid membranes of Arabidopsis plants.},
  author={Yang-Er Chen and Shu Yuan and Wolfgang P Schr{\"o}der},
  journal={Physiologia plantarum},
  year={2016},
  volume={156 1},
  pages={
          3-12
        }
}
Robust and reproducible methods for extracting thylakoid membranes are required for the analysis of photosynthetic processes in higher plants such as Arabidopsis. Here, we compare three methods for thylakoid extraction using two different buffers. Method I involves homogenizing the plant material with a metal/glass blender; method II involves manually grinding the plant material in ice-cold grinding buffer with a mortar and method III entails snap-freezing followed by manual grinding with a… CONTINUE READING
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