Comparison of methods based on annexin-V binding, DNA content or TUNEL for evaluating cell death in HL-60 and adherent MCF-7 cells.

@article{Bino1999ComparisonOM,
  title={Comparison of methods based on annexin-V binding, DNA content or TUNEL for evaluating cell death in HL-60 and adherent MCF-7 cells.},
  author={G Del Bino and Zbigniew Darzynkiewicz and Chantal Degraef and Roger Mosselmans and Dominique Fokan and Paul Galand},
  journal={Cell proliferation},
  year={1999},
  volume={32 1},
  pages={25-37}
}
HL-60 and MCF-7 cells were treated with 0.15 microM camptothecin (CPT) or with the solvent dimethylsulfoxide (DMSO) for the controls, for 2, 3 and 4 h or for 24, 48 and 72 h, respectively. The apoptotic index (AI) was then evaluated in parallel by the following flow cytometric methods: (1) double staining of unfixed cells with fluoresceinated annexin V and propidium iodide (PI), this after detachment by trypsinization in the case of MCF-7 cultures; (2) prefixation in 70% ethanol, extraction of… CONTINUE READING