Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat

@inproceedings{Liu2009ComparisonOL,
  title={Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat},
  author={Li Liu and Tatsuya M. Ikeda and G{\'e}rard Branlard and Roberto Javier Bautista Pe{\~n}a and William Chapman Rogers and Silvia Elena Lerner and Mar{\'i}a A Kolman and Xianchun Xia and Linhai Wang and Wujun Ma and Rudi Appels and Hisashi Yoshida and Aili Wang and Yueming Yan and Zhonghu He},
  booktitle={BMC Plant Biology},
  year={2009}
}
BackgroundLow-molecular-weight glutenin subunits (LMW-GS) play a crucial role in determining end-use quality of common wheat by influencing the viscoelastic properties of dough. Four different methods - sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), two-dimensional gel electrophoresis (2-DE, IEF × SDS-PAGE), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and polymerase chain reaction (PCR), were used to characterize the LMW-GS… CONTINUE READING
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