Comparison of loop-mediated isothermal amplification (LAMP) and real-time PCR method targeting a 529-bp repeat element for diagnosis of toxoplasmosis.

@article{Lin2012ComparisonOL,
  title={Comparison of loop-mediated isothermal amplification (LAMP) and real-time PCR method targeting a 529-bp repeat element for diagnosis of toxoplasmosis.},
  author={Zhibing Lin and Yanlei Zhang and Houshuang Zhang and Yongzhi Zhou and Jie Cao and Jinlin Zhou},
  journal={Veterinary parasitology},
  year={2012},
  volume={185 2-4},
  pages={
          296-300
        }
}
Loop-mediated isothermal amplification (LAMP) is a simple method that can amplify DNA with high specificity, sensitivity, and rapidity. In this study, we compared the performance of LAMP and real-time PCR assays for diagnosis of toxoplasmosis. We designed a real-time PCR assay targeting a 529 bp element repeated 200-300 times in the Toxoplasma gondii genome. The detection limits of the LAMP and real-time PCR assays were 10 fg/μL and 1 fg/μL of T. gondii DNA, respectively. Conventional PCR, LAMP… CONTINUE READING
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