Comparison of line‐field confocal optical coherence tomography images with histological sections: Validation of a new method for in vivo and non‐invasive quantification of superficial dermis thickness

  title={Comparison of line‐field confocal optical coherence tomography images with histological sections: Validation of a new method for in vivo and non‐invasive quantification of superficial dermis thickness},
  author={M{\'e}lanie Pedrazzani and Josselin Breugnot and Pauline Rouaud-Tinguely and Maxime Cazalas and Arthur J. Davis and Sylvie Bordes and Arnaud Dubois and Brigitte Closs},
  journal={Skin Research and Technology},
  pages={398 - 404}
Line‐field confocal optical coherence tomography (LC‐OCT) is an imaging technique providing “optical biopsies” of the skin in real time and non‐invasively. At a center optical wavelength of 1.3 µm, this innovative technology can be applied to dermo‐cosmetic product development due to both high image resolution (~2 µm) and sufficient penetration (~0.5 mm). Nevertheless, the precise dermal area analyzed with LC‐OCT has never been identified. In this study, the objective was to compare LC‐OCT… Expand
A plea for standardization of confocal microscopy and optical coherence tomography parameters to evaluate physiological and para‐physiological skin conditions in cosmetic science
A validated standard methodology and a list of defined parameters for objective non‐pathological skin assessments in the cosmetically sensitive cheekbone area of the face are proposed to represent a starting point for a standardized application of RCM and OCT in dermo‐cosmetic research and practice. Expand
Line‐field confocal optical coherence tomography—Practical applications in dermatology and comparison with established imaging methods
  • C. Ruini, S. Schuh, E. Sattler, J. Welzel
  • Medicine, Computer Science
  • Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging
  • 2020
While OCT is mainly indicated for detecting non‐melanoma skin cancer, RCM has proven its usefulness additionally in distinguishing melanocytic lesions, and Line‐field confocal optical coherence tomography (LC‐OCT) is an emerging tool combining the principles of both above‐mentioned methods. Expand
Applications and future directions for optical coherence tomography in dermatology *
This work aims to equip the practising dermatologist with an understanding of the principles of skin OCT and the potential clinical indications, and discusses the different modalities of OCT including angiographic (dynamic) OCT, which can image cutaneous blood vessels at high resolution. Expand
Line-field confocal optical coherence tomography for three-dimensional skin imaging
This paper reports on the latest advances in line-field confocal optical coherence tomography (LC-OCT), a recently invented imaging technology that now allows the generation of either horizontal (x ×Expand
In-Vivo LC-OCT Evaluation of the Downward Proliferation Pattern of Keratinocytes in Actinic Keratosis in Comparison with Histology: First Impressions from a Pilot Study
LC-OCT is potentially able to classify AKs based on the basal growth pattern of keratinocytes, in-vivo reproducing the PRO classification, with strong interobserver agreement and a good correlation with histopathology. Expand
In vivo characterization of healthy human skin with a novel, non‐invasive imaging technique: line‐field confocal optical coherence tomography
Line‐field confocal optical coherence tomography (LC‐OCT) is a non‐invasive optical technique recently developed for skin examination in vivo. It provides real‐time, high‐resolution vertical imagesExpand
Imaging Inflammation – From Whole Body Imaging to Cellular Resolution
The selected (3D) imaging modalities are collected and the recent findings on preclinical and clinical inflammation imaging are evaluated and the key challenges and opportunities are presented. Expand
In vivo examination of healthy human skin after short-time treatment with moisturizers using confocal Raman spectroscopy and optical coherence tomography: Preliminary observations.
  • C. Ruini, B. Kendziora, +5 authors D. Hartmann
  • Medicine
  • Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging
  • 2021
Short time moisturizing appears insufficient to provide significant changes in skin morphology and composition, as assessed by OCT and RS, and novel noninvasive imaging methods are suitable for the evaluation of skin response to topical moisturizers. Expand
Konfokale Line-Field-OCT
Die optische Kohärenztomographie (OCT) und die konfokale Lasermikroskopie (KLM) sind in der klinischen dermatologischen Routinediagnostik bereits etablierte nichtinvasive Methoden. Während die KLMExpand


Line-field confocal optical coherence tomography for high-resolution noninvasive imaging of skin tumors
The use of LC-OCT as an adjunct tool in medical practice could significantly improve clinical diagnostic accuracy while reducing the number of biopsies of benign lesions. Expand
Optical coherence tomography in dermatology
Optical coherence tomography enables evaluation of skin lesions, especially nonmelanoma skin cancers and inflammatory diseases, quantification of skin changes, visualization of parasitic infestations, and examination of other indications such as the investigation of nails. Expand
Validation of optical coherence tomography in vivo using cryostat histology.
OCT using the line-traced image analysis of the B-scan appears to be a valid and relatively practicable method for the determination of ET in vivo and the comparisons with the in vivo OCT profiles demonstrate that cryostat sectioning provides a better preservation of relative and absolute dimensions of skin layers than paraffin embedding. Expand
Optical coherence tomography in dermatology.
  • J. Olsen, L. Themstrup, G. Jemec
  • Medicine, Materials Science
  • Giornale italiano di dermatologia e venereologia : organo ufficiale, Societa italiana di dermatologia e sifilografia
  • 2015
OCT research in dermatology has undergone significant developments since its first application in 1997 and the rising demand for non-invasive monitoring of skin disorders and treatment outcomes emphasize the need for further advances in the field of non- invasive imaging. Expand
Skin-Layer Analysis Using Optical Coherence Tomography (OCT)
In the past decade, advances in optics, fiber as well as laser technology, have enabled development of a novel noninvasive optical biomedical imaging technique, optical coherence tomography (OCT).Expand
Comparison of histometric data obtained by optical coherence tomography and routine histology.
A strong correlation and low bias observed and an unsatisfactory numerical agreement demonstrated between the two OCT methods and routine histology indicating that these methods cannot be employed interchangeably are shown. Expand
High-definition optical coherence tomography intrinsic skin ageing assessment in women: a pilot study
HD-OCT allows 3-D in vivo and real time qualitative and quantitative assessment of chronological (intrinsic) age-related morphological skin changes at high resolution from skin surface to a depth of the superficial reticular dermis. Expand
Noninvasive diagnosis in dermatology
  • J. Welzel, S. Schuh
  • Medicine
  • Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG
  • 2017
In addition to dermoscopy, there are other imaging and biophysical methods for the noninvasive diagnosis of skin lesions and these methods are used in the differentiation of dysplastic nevi from melanoma. Expand
Virtual skin biopsy by optical coherence tomography: the first quantitative imaging biomarker for scleroderma
Comparison of OCT images with skin histology indicated a progressive loss of visualisation of the dermal–epidermal junction associated with dermal fibrosis, suggesting OCT of the skin could offer a feasible and reliable quantitative outcome measure in SSc. Expand
Characterization of age-related effects in human skin: A comparative study that applies confocal laser scanning microscopy and optical coherence tomography.
Comparison of CLSM and OCT suggests that the same fibrous layer is imaged by the two techniques, and this layer might be due to the transition between the papillary and reticular dermis. Expand