Comparison of kinetic models for analysis of pyruvate-to-lactate exchange by hyperpolarized 13 C NMR.

@article{Harrison2012ComparisonOK,
  title={Comparison of kinetic models for analysis of pyruvate-to-lactate exchange by hyperpolarized 13 C NMR.},
  author={Crystal E Harrison and Chendong Yang and Ashish Jindal and Ralph J DeBerardinis and Mohsen Hooshyar and Matthew E Merritt and A Dean Sherry and Craig Malloy},
  journal={NMR in biomedicine},
  year={2012},
  volume={25 11},
  pages={1286-94}
}
The activity of specific enzyme-catalyzed reactions may be detected in vivo by (13) C NMR of hyperpolarized (HP) substrates. The signals from HP substrates and products, acquired over time, have been fitted to a number of different mathematical models to determine fluxes, but these models have not been critically compared. In this study, two-pool and three-pool first-order models were constructed to measure flux through lactate dehydrogenase in isolated glioblastoma cells by NMR detection of… CONTINUE READING