Comparison of intrinsic metabolic clearance in fresh and cryopreserved human hepatocytes.

@article{Floby2009ComparisonOI,
  title={Comparison of intrinsic metabolic clearance in fresh and cryopreserved human hepatocytes.},
  author={Eva Floby and Jenny G Johansson and Janet Hoogstraate and Nicola J. Hewitt and James Hill and Anna-Karin Sohlenius-Sternbeck},
  journal={Xenobiotica; the fate of foreign compounds in biological systems},
  year={2009},
  volume={39 9},
  pages={656-62}
}
1. We compared the intrinsic clearance (CL(int)) of a number of substrates in suspensions of fresh and cryopreserved human hepatocytes from seven donors. 2. CL(int) values for a cocktail incubation of phenacetin, diclofenac, diazepam, bufuralol, midazolam, and hydroxycoumarin were 4.9 +/- 3.4, 18 +/- 7.2, 5.1 +/- 4.9, 6.3 +/- 3.3, 9.8 +/- 5.8 and 22 +/- 14 microl min(-1)/10(6) cells, respectively, and they correlated well with corresponding CL(int) values using cryopreserved hepatocytes from 25… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 10 extracted citations

Amide hydrolysis of a novel chemical series of microsomal prostaglandin E synthase-1 inhibitors induces kidney toxicity in the rat.

Drug metabolism and disposition: the biological fate of chemicals • 2013
View 4 Excerpts
Highly Influenced

Cytochrome p450 inhibitory properties of common efflux transporter inhibitors.

Drug metabolism and disposition: the biological fate of chemicals • 2014
View 1 Excerpt

Novel bioactivation mechanism of reactive metabolite formation from phenyl methyl-isoxazoles.

Drug metabolism and disposition: the biological fate of chemicals • 2012
View 1 Excerpt

References

Publications referenced by this paper.
Showing 1-10 of 19 references

The role of hepatic transporters in drug elimination.

Expert opinion on drug metabolism & toxicology • 2008
View 2 Excerpts

Similar Papers

Loading similar papers…